TABLE 2

Transposition frequencies of {GAL4Δ}, {GAL4Δ + UAS}, and {tTA} mutator elements

Mutator elementG2 crossesaJumping rateb (%)Enhancer detectionc (%)Stable new insertionsd (%)Σ maleseTransposition frequencyfExcision rateg (%)
{GAL4 Δ } 23792 (81–100)476711,4050.123 ± 0,03196–100
{GAL4Δ + UAS}22572 (36–96)51459,1530.084 ± 0,04848–100
{tTA} 22780 (60–96)2419,5000.130 ± 0,05556–100

For each mutator element, three independent homozygous strains were crossed to three independent homozygous jumpstarter strains (nine combinations as in Figure 1, G1 cross): Dm[{GAL4Δ}] strains: M8-2x, M19-2x, M19-3x. Dm[{GAL4Δ + UAS}] strains: M5.x, FM26.x, FF36.x. Dm[{tTA}] strains: x.2, x.5, x.7. Dm[Her{3xP3-ECFP, αtub-piggyBacK10}] jumpstarter strains: M1.III, M6.II, M10.III. Per combination, 27 single male crosses were set up as shown in Figure 1 (G2 cross).

  • a Total number of fertile single male G2 crosses (of 243 crosses per mutator).

  • b Jumping rate is the percentage of (G2 crosses with at least one 3xP3-EYFP+ son)/(fertile G2 crosses) (Berg and Spradling 1991); the mean value and the range of nine independent combinations are indicated.

  • c Percentage of (G2 crosses with at least one 3xP3-EYFP+ son detecting an adult enhancer activity)/(fertile G2 crosses).

  • d Percentage of [G2 crosses with at least one 3xP3-EYFP+ and 3xP3-ECFP (no jumpstarter) son]/(fertile G2 crosses).

  • e Total number of G3 males.

  • f Transposition frequency [(Σ 3xP3-EYFP+ males)/(Σ males)] × 9/4 (Berg and Spradling 1991), since only four of nine mutated chromosome arms have been recovered. Standard error of the mean is indicated.

  • g Excision rate is the percentage of (G2 crosses with at least one 3xP3-EYFP daughter)/(fertile G2 crosses), the range of nine independent combinations is indicated.