TABLE 1

Summary of generated jumpstarter, mutator, and reporter constructs

Jumpstarterhsp70-Hermeshsp 70-piggyBac α tub-piggyBac
ProgenitorspSLfa_hsp70-Hermes_fapSLfa_hsp70-piggyBac_fapSLfa_αtub-piggyBacK10_fa
Final constructspBac{3xP3-ECFP, hsp70-Hermes}apHer{3xP3-ECFP, hsp70-piggyBac}pHer{3xP3-ECFP, αtub-piggyBacK10}a
pMi{3xP3-DsRed, hsp70-piggyBac}apHer{3xP3-EYFP, αtub-piggyBacK10}
pMos{3xP3-ECFP, αtub-piggyBacK10}a
pMos{3xP3-EYFP, αtub-piggyBacK10}
Mutator{GAL4 Δ }{GAL4Δ+UAS}{tTA}{tTA+TRE}
ProgenitorspSLfa_p-GAL4Δ-K10_fapSLfa_p-GAL4Δ-K10_fapSLfa_p-tTA-K10_fapSLfa_p-tTA-K10_fa
pBac{UASp-3xP3-EYFPafm}pBac{TRE-3xP3-EYFPafm}
Final constructspHer{3xP3-EYFP, p-GAL4Δ-K10}pBac{UASp-3xP3-EYFP, p-GAL4Δ-K10}apBac{3xP3-EYFP, p-tTA-K10}apBac{TRE-3xP3-EYFP, p-tTA-K10}a
pBac{3xP3-EYFP, p-GAL4Δ-K10}a
ReporterUASp-EYFPUASp-lacZTRE-EYFPTRE-lacZ
ProgenitorspSLfa_UASp-eyfp-K10_fapSLfa_UASp-lacZ-K10_fapSLfa_TRE-EYFP-K10_fapSLfa_TRE4acZ-K10_fa
Final constructspBac{3xP3-ECFP, UASp-EYFP-K10}pBac{3xP3-ECFP, UASp-lacZ-K10}apBac{3xP3-ECFP, TRE-EYFP-SV40}apBac{3xP3-ECFP, TRE4acZ-SV40}a
pBac{3xP3-EYFP, UASp-EYFP-K10}apBac{3xP3-EYFP, UASp-lacZ-K10}pBac{3xP3-DsRed, TRE-EYFP-SV40}apBac{3xP3-DsRed, TRE-lacZ-SV40}
pBac{3xP3-DsRed, UASp-EYFP-K10}apBac{3xP3-DsRed, UASp-lacZ-K10}
pHer{3xP3-ECFP, UASp-EYFP-K10}apHer{3xP3-ECFP, UASp-lacZ-K10}a
pHer{3xP3-EYFP, UASp-EYFP-K10}pHer{3xP3-EYFP, UASp-lacZ-K10}
  • a Constructs have been proven functional in transgenic Drosophila strains.