TABLE 2

Primers used for amplification and sequencing of the GHR exons from bovine genomic DNA

GHRex3_FTAG GAG TTC CTT TTA GAG GAT AGG TGC
GHRex3_RGCC TTG TGG AGA AGT TGA CAA A
GHRex4_FGCC CAG AGA AAC AGC ATT TCT A
GHRex4_RTCA CTG CCA TAT TTC CAG CAT C
GHRex5_FCTT GCT CAT AAA ATA CTC GTG TCC T
GHRex5_RATG CAA TGG CAA AGT CTT CCT AC
GHRex6_FTGT ATG AAG TAA CTT AGT CGT CTT CG
GHRex6_RGAG AGG GGT TGT TGA ACA CAA A
GHRex7_FTCC TAC TTT CCA GAA ATT CAT TTT G
GHRex7_RCTG AGG CTA ATG TAT ATT GAT CTG GAC
GHRex8_FGTG GCT ATC AAG TGA AAT CAT TGA C
GHRex8_RACT GGG TTG ATG AAA CAC TTC ACT C
GHRex9_FGCC TCA TCA TTC ACT GCT TA
GHRex9_RGGT TTC AAC ATA AGG CTC TG
GHRex10_FACA TGG TTT GTT ATA TGA TTT TGT TAC
GHRex10_RTTC ATA TTC CCC ACC CTC AAC T
GHRex10_1FACA TTC TGG AGG CTG ATT TC
GHRex10_2FCAA AAG AAT AAG ACT GGG AA
GHRex10_1RAGC TTG GCT CTA CGT GTG AT
GHRex10_2RGAT AAC ACT GGG CTG CTG GT
  • All primer sequences are written 5′ → 3′. All exons were PCR amplified and sequenced with the same primers except for exon 10, which was amplified with GHRex10_F and GHRex10_R and then sequenced with these primers plus GHRex10_1F, GHRex10_1R, GHRex10_2F, and GHRex10_2R.