TABLE 2

CDC42 interaction-enhancing mutations

Template mutationAdditional mutations
N39SP69T (2)
N39SG60S (2)
N39SG12S
N39SG12H
N39SY64H
N39SD65G
P69TN39S (3)
P69TN39S, G12S
P69TY64H
  • Mutations that enhanced the two-hybrid interaction be- tween Cdc42p and the Ste20p CRIB domain containing the H345D substitution were identified after mutagenesis of tem- plates containing either the N39S or the P69T mutations. The most frequent event reconstructed the N39S P69T double mutation, but other changes were also identified.