TABLE 1

Yeast strains

StrainGenotypeSource
W303-1Aade2 ura3 his3 leu2 trp1 can1R. Rothstein
JAY25W303-1A with ste20H348DThis study
JAY26W303-1A with ste20H345DThis study
JAY39JAY25 with sst1::ura3 fus1::LacZ::URA3This study
JAY40JAY26 with sst1::ura3 fus1::LacZ::URA3This study
JAY54W303-1A with sst1::ura3 fus1::LacZ::URA3This study
DM98001W303a/α but cla4::TRP1/+ and ste20H345D/+This study
DM98014W303a/α but cla4::TRP1/+ and ste20H348D/+This study
EL254-1BW303-1B with cla4::TRP1This study
M98001-2AW303-1A cla4::TRP1ste20H345D(pSTE20::URA3)This study
M98014-1AW303-1A cla4::TRP1ste20H348D(pSTE20::URA3)This study
YCW563W303-1A sst1::hisGThis study
    With the following plasmids:
        pRL116C.4 (STE20:GFP)
        pJA82 (ste20H345D:GFP)
        pJA83 (ste20H348D:GFP)
        pRS315:CDC42 pJA55 (CDC42N39S:P69T)