Actin localization defect in cdc5 mutants
CDC5 CDC14TAB6-1 | cdc5-1 CDC14TAB6-1 | cdc5-3 CDC14TAB6-1 | cdc5-4 CDC14TAB6-1 | cdc5-7 CDC14TAB6-1 | |
---|---|---|---|---|---|
% cells with localized actin | 87.1 | 75.9 | 56.1 | 54.3 | 46.1 |
Cells were grown at 23° and then shifted to 37° for 3.5 hr prior to fixation. After sonication, ̃200 cells with elongated spindles were counted to determine the percentage of cells with localized actins at the bud neck. CDC5 CDC14TAB6-1, KLY2970; cdc5-1 CDC14TAB6-1, KLY2946; cdc5-3 CDC14TAB6-1, KLY2950; cdc5-4 CDC14TAB6-1, KLY2954; cdc5-7 CDC14TAB6-1, KLY2958.