TABLE 3

Actin localization defect in cdc5 mutants

CDC5
CDC14TAB6-1
cdc5-1
CDC14TAB6-1
cdc5-3
CDC14TAB6-1
cdc5-4
CDC14TAB6-1
cdc5-7
CDC14TAB6-1
% cells with localized actin87.175.956.154.346.1
  • Cells were grown at 23° and then shifted to 37° for 3.5 hr prior to fixation. After sonication, ̃200 cells with elongated spindles were counted to determine the percentage of cells with localized actins at the bud neck. CDC5 CDC14TAB6-1, KLY2970; cdc5-1 CDC14TAB6-1, KLY2946; cdc5-3 CDC14TAB6-1, KLY2950; cdc5-4 CDC14TAB6-1, KLY2954; cdc5-7 CDC14TAB6-1, KLY2958.