TABLE 2

Induction of aberrant septin ring structures in the cdc5-4 and cdc5-7 mutants occurs prior to mitotic exit

CDC5CDC5
CDC14TAB6-1
cdc5-4cdc5-4
CDC14TAB6-1
cdc5-7cdc5-7
CDC14TAB6-1
% asynchronous0.00.035.39.554.220.8
% α → release0.00.070.335.880.347.2
% α → noc0.00.00.80.52.15.2
  • To prepare randomly growing samples, cells grown at 23° were shifted to 37° for 4 hr prior to fixation. For α-factor-treated samples, cells were cultured in the presence of α-factor for 2.5 hr at 23° and then released into either prewarmed fresh medium or nocodazole-containing medium for an additional 4 hr at 37°. Both α-factor and nocodazole were used at the concentration of 15 μg/ml. CDC5, KLY3209; CDC5 CDC14TAB6-1, KLY3075; cdc5-4, KLY3205; cdc5-4 CDC14TAB6-1, KLY3071; cdc5-7, KLY3206; cdc5-7 CDC14TAB6-1, KLY3072.