Induction of aberrant septin ring structures in the cdc5-4 and cdc5-7 mutants occurs prior to mitotic exit
| CDC5 | CDC5 CDC14TAB6-1 | cdc5-4 | cdc5-4 CDC14TAB6-1 | cdc5-7 | cdc5-7 CDC14TAB6-1 | |
|---|---|---|---|---|---|---|
| % asynchronous | 0.0 | 0.0 | 35.3 | 9.5 | 54.2 | 20.8 |
| % α → release | 0.0 | 0.0 | 70.3 | 35.8 | 80.3 | 47.2 |
| % α → noc | 0.0 | 0.0 | 0.8 | 0.5 | 2.1 | 5.2 |
To prepare randomly growing samples, cells grown at 23° were shifted to 37° for 4 hr prior to fixation. For α-factor-treated samples, cells were cultured in the presence of α-factor for 2.5 hr at 23° and then released into either prewarmed fresh medium or nocodazole-containing medium for an additional 4 hr at 37°. Both α-factor and nocodazole were used at the concentration of 15 μg/ml. CDC5, KLY3209; CDC5 CDC14TAB6-1, KLY3075; cdc5-4, KLY3205; cdc5-4 CDC14TAB6-1, KLY3071; cdc5-7, KLY3206; cdc5-7 CDC14TAB6-1, KLY3072.