TABLE 1

Strains used in this study

StrainsGenotype
KLY1546aMATa his3-11, 15 leu2-3, 112 trp1-1 ura3
KLY1548MATα KLY1546
KLY2372KLY1546 cdc5Δ::KanMX6 LEU2::TUB1-GFP + YCplac33-CDC5
KLY2458KLY1546 LEU2::TUB1-GFP cdc5Δ::KanMX6 TRP1::cdc5-1-HA3
KLY2460KLY1546 LEU2::TUB1-GFP cdc5Δ::KanMX6 TRP1::cdc5-3-HA3
KLY2462KLY1546 LEU2::TUB1-GFP cdc5Δ::KanMX6 TRP1::cdc5-4-HA3
KLY2464KLY1546 LEU2::TUB1-GFP cdc5Δ::KanMX6 TRP1::cdc5-7-HA3
KLY2470KLY1546 LEU2::TUB1-GFP cdc5Δ::KanMX6TRP1::CDC5-HA3
KLY2946KLY2458 HIS3::CDC14TAB6-1
KLY2950KLY2460 HIS3::CDC14TAB6-1
KLY2954KLY2462 HIS3::CDC14TAB6-1
KLY2958KLY2464 HIS3::CDC14TAB6-1
KLY2970KLY2470 HIS3::CDC14TAB6-1
KLY4130MATa/α KLY2946/KLY1548
KLY4131MATa/α KLY2950/KLY1548
KLY4132MATa/α KLY2954/KLY1548
KLY4133MATa/α KLY2958/KLY1548
KLY3076KLY1546 cdc5Δ::KanMX6 TRP1::cdc5-3-HA3 HIS3::CDC14TAB6-1 swe1Δ::LEU2
KLY3122KLY1546 cdc5Δ::KanMX6 TRP1::cdc5-4-HA3 HIS3::CDC14TAB6-1 swe1Δ::LEU2
KLY3155KLY1546 cdc5Δ::KanMX6 TRP1::cdc5-7-HA3 HIS3::CDC14TAB6-1 swe1Δ::LEU2
KLY3080KLY1546 cdc5Δ::KanMX6 TRP1::cdc5-3-HA3 HIS3::CDC14TAB6-1
KLY2868KLY1546 hsl1Δ::URA3
KLY3170KLY3080 hsl1Δ::URA3
KLY3173KLY3080 hsl1Δ::URA3 swe1Δ::LEU2
KLY3071KLY2954 URA3::YFP-CDC10
KLY3072KLY2958 URA3::YFP-CDC10
KLY3075KLY2970 URA3::YFP-CDC10
KLY3205KLY2462 URA3::YFP-CDC10
KLY3206KLY2464 URA3::YFP-CDC10
KLY3209KLY2470 URA3::YFP-CDC10
KLY3578KLY1546 cdc5Δ::KanMX6 TRP1::cdc5-7-HA3 HIS3::CDC14TAB6-1 LEU2::YFP-CDC10
KLY2374KLY1546 bfa1Δ::his5+
KLY2573KLY1546 bub2Δ::his5+
KLY3826KLY1546 cdc5Δ::KanMX6 URA3::YFP-CDC10 TRP1::cdc5-1-HA3 bfa1Δ::his5+
KLY3831KLY1546 cdc5Δ::KanMX6 URA3::YFP-CDC10 TRP1::cdc5-1-HA3 bub2Δ::his5+
KLY3828KLY1546 cdc5Δ::KanMX6 URA3::YFP-CDC10 TRP1::cdc5-3-HA3 bfa1Δ::his5+
KLY3832KLY1546 cdc5Δ::KanMX6 URA3::YFP-CDC10 TRP1::cdc5-3-HA3 bub2Δ::his5+
KLY3829KLY1546 cdc5Δ::KanMX6 URA3::YFP-CDC10 TRP1::cdc5-4-HA3 bfa1Δ::his5+
KLY3834KLY1546 cdc5Δ::KanMX6 URA3::YFP-CDC10 TRP1::cdc5-4-HA3 bub2Δ::his5+
KLY3371KLY1546 cdc5Δ::KanMX6 URA3::YFP-CDC10 TRP1::cdc5-7-HA3 bfa1Δ::his5+
KLY3375KLY1546 cdc5Δ::KanMX6 URA3::YFP-CDC10 TRP1::cdc5-7-HA3 bub2Δ::his5+
  • a KLY1546 is in the W303-1A genetic background.