TABLE 4

glp-1(ar202) maternal effects

GenotypeProgeny germline pattern
MaternalPaternalProgeny% wild type% Pro% other(n)
ar202/ar202a(Self)ar202/ar20210900(129)
ar202/+b(Self)ar202/ar202c76213(63)
ar202/ar202d(Self)ar202/ar202d165231(61)
ar202/ar202/+e(Self)ar202/ar202f13843(75)
ar202/ar202g+/+ar202/+h431442(65)
+/+iar202/ar202ar202/+j9712(76)
  • a Actual genotype: glp-1(ar202) unc-69(e587).

  • b Actual genotype: glp-1(ar202) unc-69(e587)/eT1.

  • c Actual genotype: glp-1(ar202) unc-69(e587). “% other”: Class A.

  • d Actual genotype: dpy-17(e164) glp-1(ar202). “% Pro” includes 3% Pro + Class B. All “% other” display Class A; half of these display Class A + Class B. As an additional control for the other markers present in the duplication-bearing strain, self-progeny from mothers of the genotype dpy-17(e164) ncl-1(e1865) unc-36(e251) glp-1(ar202) were also scored (n = 65) and they displayed penetrance of 34% wild type and 66% Pro (the “% other” classes were not determined in this experiment).

  • e Actual genotype: dpy-17(e164) ncl-1(e1865) unc-36(e251) glp-1(ar202); qDp3.

  • f Actual genotype: dpy-17(e164) ncl-1(e1865) unc-36(e251) glp-1(ar202). “% Pro” includes 27% Pro + Class B. “% other”: Class A.

  • g Actual genotype: unc-32(e189) glp-1(ar202).

  • h Actual genotype: unc-32(e189) glp-1(ar202)/++ [identified as non-Unc F1 cross-progeny of wild-type males mated with unc-32(e189) glp-1(ar202) hermaphrodites]. “% Pro” includes 12% Pro + Class B. “% other” includes 2% Class A, 35% Class A + Class B, and 5% Class B alone. Cross-progeny were synchronized by removing the parents after an overnight egg laying. Eggs and L1s were shifted to the restrictive temperature and scored as described in materials and methods.

  • i Actual genotype: unc-32(e189).

  • j Actual genotype: glp-1(ar202)/unc-32(e189) [identified as non-Unc F1 cross-progeny of glp-1(ar202) males mated with unc-32(e189) hermaphrodites]. The Pro animal was Pro + Class B. “% other”: Class A. Temperature- shift protocol was used as in note h above.