TABLE 5

Effect of histone H3 and H4 N-terminal tail mutations on yFACT mutants

Histone tail
H3H4WTspt16-11pob3-L78Rnhp6a/b
WTWT++++++++++
Δ3-29WT+++---
WTΔ4-19+++-+/--
K9QWT++++++++++
K9RWT++++++++++
K14RWT++++++++++
WTK5,12R+++-++++
WTK5,12Q++++++++++
WTK8, 16R++++--
WTK8,16Q++++++++++
K14QK8,16Q++++++++++
  • Strains DY7803 (WT), DY7809 (spt16-11), DY7818 (pob3-L78R), and DY7142 (nhp6a/b-Δ) carrying YCp50-HHT2-HHF2 were transformed with plasmids with the mutations indicated (Zhang et al. 1998) and then tested on medium containing 5-FOA for ability to grow at 34° (spt16-11), 25° (pob3-L78R), or 30° (nhp6a/b-Δ). The sizes of individual colonies were assessed after several days and compared to WT (defined to be +++). WT, wild type.