TABLE 3

Glucose incorporation into 1,3-β-glucan in fks1-1154 cells with the multicopy suppressor genes at 34°

Strain (plasmid)Relative incorporation
into 1,3-β-glucan
fks1-1154 (pYO326)1.00 ± 0.02
fks1-1154 (YEpU-MID2)1.11 ± 0.11
fks1-1154 (YEpU-WSC1)1.36 ± 0.23
fks1-1154 (YEpU-WSC3)1.70 ± 0.45
fks1-1154 (YEpU-MTL1)1.69 ± 0.47
fks1-1154 (YEpU-ROM2)1.44 ± 0.26
fks1-1154 (YEpU-LRE1)1.47 ± 0.52
fks1-1154 (YEpU-ZDS1)1.51 ± 0.06
fks1-1154 (YEpU-MSB1)1.56 ± 0.14
fks1-1154 [YCpL-RHO1(Q68L)]2.67 ± 0.43
fks1-1154 [YCpL-RHO1(G19V)]1.85 ± 0.26
FKS1 (pYO326)3.06 ± 0.69
  • Each datum represents the means and SD of at least three experiments.