TABLE 2

Plasmids used in this study

NameParent plasmidMarkersReference
pJJ217pUC18HIS3Jones and Prakash (1990)
pJJ283pUC18LEU2Jones and Prakash (1990)
pRS316URA3, CENSikorski and Hieter (1989)
pYO971pRS316URA3, CENThis study
pYO326pRS306URA3, 2μ originQadota et al. (1994)
pYO326NSpYO326URA3, 2μ originThis study
pYO701pRS314TRP1, RHO1 promoter, RHO1 terminator, CENQadota et al. (1994)
pYO962 [YCpL-RHO1(Q68L)]pRS315LEU2, RHO1(Q68L), CENThis study
pYO964 [YCpU-RHO1(G19V)]pRS316URA3, RHO1(G19V), CENThis study
pYO965 [YCpU-RHO1(Q68L)]pRS316URA3, RHO1(Q68L), CENThis study
pYO1835 (YEpU-RHO1)pYO326URA3, RHO1, 2μ originThis study
pYO2325 (YEpU-MTL1)pYO326URA3, MTL1, 2μ originThis study
pYO2358 (YEpU-FKS1)pYO326URA3, FKS1, 2μ originThis study
pYO2361 (YEpU-MID2)pYO326URA3, MID2, 2μ originThis study
pYO2364 (YEpU-WSC1)pYO326URA3, WSC1, 2μ originThis study
pYO2366 (YEpU-ROM2)pYO326URA3, ROM2, 2μ originThis study
pYO2368 (YEpU-LRE1)pYO326URA3, LRE1, 2μ originThis study
pYO2369 (YEpU-WSC3)pYO326URA3, WSC3, 2μ originThis study
pYO2370 (YEpU-ZDS1)pYO326URA3, ZDS1, 2μ originThis study
pYO2371 (YEpU-MSB1)pYO326URA3, MSB1, 2μ originThis study