TABLE 2

Two-hybrid analysis of GAD-Sir2p/Gbd-Esc2p or Gbd-Esc8p interactions

Class I
mutants
Esc2pEsc8pClass II
mutants
Esc2pEsc8p
Sir2-12p++++++Sir2-81p++++++
Sir2-143p++++++Sir2-82p++++++
Sir2-146p++++++Sir2-85p++++++
Sir2-213p++++++Sir2-86p++++++
Sir2-424p++++++Sir2-88p--
Sir2-512p++++++Sir2-89p++++++
Sir2-93p--
Sir2-131p--
ControlsSir2-167p--
    Sir2p++++++Sir2-199p--
    Vector--Sir2-87p--
  • -, no growth on SC-His plates after 5 days; +++, growth on SC-His + 10 mm aminotriazole and on SC-Ade plates.