TABLE 4

Gene conversion rates in wild-type and rad strains containing the GCR substrates (recipient highly transcribed)

Low txn (Gal80+)High txn (Gal80-)
RAD genotypeRate (×10-8)NormalizedaRate (×10-8)NormalizedaHigh txn/low txn
Wild type5.501.096.117.517.5
(4.75-6.07)(87.6-109)
rad5046.08.3616730.43.63
(38.8-50.3)(149-187)
rad510.300.051.640.305.47
(0.24-0.35)(1.31-2.10)
rad520.02NABR0.08NABRNABR
(0-0.08)(0.07-0.11)
rad540.230.040.810.153.52
(0.10-0.24)(0.71-1.12)
rad551.550.2836.66.6523.6
(1.34-1.79)(32.1-38.4)
rad572.570.4749.89.0519.4
(2.10-2.95)(48.1-53.6)
rad592.770.5067.712.324.4
(2.40-3.22)(62.1-76.7)
  • Gene conversion rates indicated as being NABR (not above background reversion) correspond to Lys+ rates that are not statistically above the background reversion rates of the lys2-oligo allele. The pGAL-lys2-oligo allele reverts at a rate of 5.1 × 10-10 in a wild-type Gal80- background and at a rate of 1.3 × 10-9 in a rad52 Gal80- background. The 95% confidence interval is given in parentheses below each rate. txn, transcription

  • a Normalized to wild type, low transcription.