TABLE 3

Gene conversion rates in wild-type and rad strains containing the GCD substrates (donor highly transcribed)

Low txn (Gal80+)High txn (Gal80-)
RAD genotypeRate (×10-8)NormalizedaRate (×10-8)NormalizedaHigh txn/low txn
Wild type3.231.021.96.786.78
(2.70-3.78)(20.9-24.5)
rad5054.817.070.521.81.29
(50.1-59.0)(66.2-74.7)
rad510.06NABR0.470.15≥7.83
(0-0.12)(0.33-0.64)
rad520.03NABR0.04NABRNABR
(0-0.07)(0-0.13)
rad540.10NABR0.340.11≥3.40
(0.06-0.16)(0.22-0.60)
rad551.950.600.960.300.49
(1.40-2.48)(0.79-1.32)
rad572.730.851.430.440.52
(1.98-3.24)(1.21-1.56)
rad592.120.6614.84.586.98
(1.86-2.63)(11.9-18.0)
  • Gene conversion rates indicated as being NABR (not above background reversion) correspond to Lys+ rates that are not statistically above the background reversion rates of the lys2-oligo allele. The pGAL-lys2-oligo allele reverts at a rate of 5.1 × 10-10 in a wild-type Gal80- background and at a rate of 1.3 × 10-9 in a rad52 Gal80- background. The 95% confidence interval is given in parentheses below each rate. txn, transcription.

  • a Normalized to wild type, low transcription.