P-element S129A transposase yields a higher frequency of P-element-mediated germline transformation

Helper DNA
No. of
fertile adults
No. of
transformant vials
% transformedbNo. of different
by Southern
blot hybridization
Total no. of
Wild type50-150 μg/mla4636.5310
S129A 50-150 μg/ml53918.91021
Wild type 250-1250 μg/ml20110.022
S129A 250-1250 μg/ml832248.24078
Wild type 50-250 μg/ml107610.31122
S129A 50-250 μg/ml741327.02037
Wild type 250-2250 μg/ml31735.51120
S129A 250-2250 μg/ml1412434.04884
  • Data are shown from two experiments using the indicated actin-transposase helper plasmid DNA concentration and the transformation vector, pw8, at 500 μg/ml. The number of fertile adults, number of transformant vials, the percentage of transformed fertile G0 adults, the number of different independent insertions as assayed by DNA blot hybridization (data not shown), and the total number of transformants obtained are indicated in the labeled columns.

  • a P-element transformation vector, pw8; concentration, 500 μg/ml.

  • b Tranformation frequency has traditionally been given as the number of G0 progeny producing one or more transformants/ the number of fertile G0 adults (Spradling 1986). However, since here we determined the number of different transformants by Southern blot hybridization, we used this number, rather than the number of G0 progeny producing at least one transformant, to calculate the transformation frequency.