TABLE 3

Statistical analysis of the capping assay data

Homologous (+TG)NHEJ (no TG)
Straina25% quartilebMedian frequency: 50% quartilec75% quartiledP value vs. wtenf% n = 0g25% quartilebMedian frequency: 50% quartilec75% quartiledP value vs. wtenf% n = 0g
Wild type00.000.211.000325600.080.231.0003244
yku70Δ12.5030.12145.000.00046000.020.290.7284850
yku80Δ16.0024.9153.500.00016000.000.080.0801675
cdc13-146.0078.93110.000.0003200.060.601.450.0063225
cdc13-1 (30°/4 hr)110.00172.94205.000.0001600.501.572.400.000166
stn1-130.330.551.250.00016130.110.360.660.0561619
stn1-13 (37°/4 hr)1.704.3512.000.00016000.221.400.1331631
tel1Δ8.1511.0019.500.00032000.000.570.4893263
rif1Δ00.040.390.54316500.140.692.450.0051619
rif2Δ00.000.190.76016630.052.193.600.0051625
sir20.050.200.470.026162500.050.200.4971650
sir300.170.290.339164400.000.030.0371675
rad5200.000.190.924325300.0000.0013281
rad51Δ0.381.465.250.000321600.160.850.5373250
rad100.130.820.12016380.080.210.350.1781613
mre11Δ0.110.260.600.000441600.000.110.0074470
rad50Δ0.030.420.850.000482500.000.140.0524860
xrs2Δ00.130.640.238165000.0000.0051688
yku70Δ tel1Δ105.00247.97400.000.0001601.653.377.800.000166
yku80Δ tel1Δ140.00173.49365.000.00016001.143.600.0341638
cdc13-1 tel1Δ31.5058.0868.500.0003201.051.502.000.000329
yku70Δ rad522.903.518.000.0001660.080.260.830.1081625
yku70Δ rad51Δ120.00164.62215.000.0001601.403.857.900.000166
yku70Δ rad11.551.385.500.00016000.000.160.0641669
yku80Δ rad521.552.823.950.00032600.320.780.1153232
yku80Δ rad51Δ245.00308.54510.000.0001601.092.403.800.000166
yku80Δ rad10.882.842.050.00016000.050.120.3641650
cdc13-1 rad523.605.8714.500.00032600.822.300.0193238
cdc13-1 rad51Δ24.0031.8950.500.0001600.370.791.350.000166
cdc13-1 rad110.9016.0127.000.0001600.100.570.870.0531619
tel1Δ rad521.202.646.300.000481300.361.400.0244835
tel1Δ rad51Δ44.5066.50140.000.00016000.140.290.9541644
tel1Δ rad11.452.864.750.0003260.000.050.470.8593250
cdc13-1 mre11Δ25.0059.30170.000.0001600.310.911.650.0021619
cdc13-1 rad5030.0054.2972.500.0001601.153.036.500.000166
cdc13-1 xrs2Δ19.2593.52165.000.0001601.60313.900.000166
tel1Δ mre11Δ1.703.5710.950.00016600.490.830.0881631
tel1Δ rad50Δ2.754.776.900.00016600.190.860.4581644
tel1Δ xrs2Δ1.553.367.200.00016600.000.480.8991656
  • a All strains are listed in the Figure 3 and 4 legends. Assays were done as described in materials and methods.

  • b The lower quartile boundary, below which lies one-quarter of the frequencies of the data set, given as per million.

  • c The raw median frequency for the data set, given as per million.

  • d The upper quartile boundary, below which lies three-quarters of the frequencies of the data set, given as per million.

  • e Probability that the mutant strain distribution is the same as wild type (described in materials and methods).

  • f Number of isolates assayed.

  • g Percentage of isolates assayed that had no capture events.