TABLE 2

Double-mutant strains used in assays

SporeMutant genotypeaParental mating
UCC2391rad52::LEU2 yku70Δ::TRP1UCC2364 × UCC2384
UCC2392rad52::LEU2 yku80Δ::TRP1UCC2365 × UCC2384
UCC2744rad52::LEU2 tel1Δ::kanMXUCC2770 × UCC2402
UCC2745tel1Δ::LEU2 yku70Δ::kanMXUCC2366 × UCC2720
UCC2746tel1Δ::LEU2 yku80Δ::kanMXUCC2367 × UCC2720
UCC2747tel1Δ::LEU2 cdc13-1UCC2769 × UCC2357
UCC2748tel1Δ::LEU2 rad50Δ::kanMXUCC2399 × UCC2720
UCC2749tel1Δ::LEU2 mre11Δ::kanMXUCC2336 × UCC2720
UCC2750tel1Δ::LEU2 xrs2Δ::kanMXUCC2461 × UCC2720
UCC2757cdc13-1 rad50Δ::kanMXUCC2492 × UCC2401
UCC2758cdc13-1 mre11Δ::kanMXUCC2374 × UCC2357
UCC2759cdc13-1 xrs2Δ::kanMXUCC2492 × UCC2473
UCC2760cdc13-1 rad52::LEU2UCC2770 × UCC2357
UCC2774rad1::LEU2 tel1Δ::kanMXUCC2421 × UCC2402
UCC2780rad51Δ::kanMX tel1Δ::LEU2UCC2769 × UCC2431
UCC2789rad51Δ::kanMX yku70Δ::TRP1UCC2364 × UCC2431
UCC2790rad51Δ::kanMX yku80Δ::TRP1UCC2365 × UCC2431
UCC2791rad51Δ::kanMX cdc13-1UCC2492 × UCC2431
UCC2809rad1::LEU2 yku70Δ::kanMXUCC2366 × UCC2771
UCC2810rad1::LEU2 yku80Δ::kanMXUCC2367 × UCC2771
UCC2811rad1::LEU2 cdc13-1UCC2421 × UCC2357
  • a All of the double-mutant strains have this base genotype: MATa-inc ade2::hisG his3Δ200 leu2Δ0 lys2Δ1::GAL-HO/LYS2 met15Δ0 trp1Δ63 ura3Δ0 can1Δ::ADE2.