TABLE 1

Wild-type, single-mutant, and diploid strains

StrainGenotypeParent
UCC2325MATa-inc ade2::hisG his3Δ200 leu2Δ0 lys2Δ1::GAL-HO/LYS2 met15Δ0 trp1Δ63 ura3Δ0Spore of UCC5843 × BY4727a
UCC2326MATa-inc ade2::hisG his3Δ200 leu2Δ0 lys2A1::GAL-HO/LYS2 met15Δ0 trp1Δ63 ura3Δ0Spore of UCC5843 × BY4727a
UCC2327MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ1::GAL-HO/LYS2 met15Δ0 trp1Δ63 ura3Δ0Spore of UCC5843 × BY4727a
UCC2330bMATa-inc ade2::hisG his3Δ200 leu2Δ0 lys2Δ1::GAL-HO/LYS2 met15Δ0 trp1Δ63 ura3Δ0 can1Δ::TRP1UCC2325
UCC2332MATα ade2::hisG his3Δ200 leu2Δ0 lys2Δ1::GAL-HO/LYS2 met15Δ0 trp1Δ63 ura3Δ0 can1Δ::TRP1UCC2327
UCC2333byku70Δ::LEU2UCC2330b
UCC2334byku80Δ::LEU2UCC2330b
UCC2335brad52::LEU2UCC2330b
UCC2336bmre11Δ::kanMXUCC2330b
UCC2338MATa-inc ade2::hisG his3Δ200 leu2Δ0 lys2Δ1::GAL-HO/LYS2 met15Δ0 trp1Δ63 ura3Δ0 can1Δ::ADE2UCC2325
UCC2339MATa-inc ade2::hisG his3Δ200 leu2Δ0 lys2Δ1::GAL-HO/LYS2 met15Δ0 trp1Δ63 ura3Δ0 can1Δ::ADE2UCC2326
UCC2356bcdc13-1UCC2330b
UCC2357cdc13-1UCC2332
UCC2364yku70Δ::TRP1UCC2339
UCC2365yku80Δ::TRP1UCC2339
UCC2366yku70Δ::kanMXUCC2338
UCC2367yku80Δ::kanMXUCC2338
UCC2370yku70Δ::LEU2UCC2332
UCC2374mre11Δ::kanMXUCC2338
UCC2384rad52::LEU2UCC2332
UCC2399brad50Δ::kanMXUCC2330b
UCC2400btel1Δ::kanMXUCC2330b
UCC2401rad50Δ::kanMXUCC2332
UCC2402tel1Δ::kanMXUCC2332
UCC2421rad1::LEU2UCC2338
UCC2430brad51Δ::kanMXUCC2330b
UCC2431rad51Δ::kanMXUCC2332
UCC2446bstn1-13UCC2330b
UCC2459brif1Δ::kanMXUCC2330b
UCC2460brif2Δ::kanMXUCC2330b
UCC2461bxrs2Δ::kanMXUCC2330b
UCC2473xrs2Δ::kanMXUCC2332
UCC2492can1Δ::ADE2UCC2356b
UCC2499MATa-inc/MATα ade2::hisG/ade2:hisG his3Δ200/his3Δ200 leu2Δ0/leu2Δ0 lys2Δ1::GAL-HO/LYS2/lys2Δ1::GAL-HO/LYS2 met15Δ0/met15Δ0 trp1Δ63/trp1Δ63 ura3Δ0/ura3Δ0 can1Δ::ADE2/can1Δ::TRP1 mre11Δ::kanMX/MRE11 YKU70/yku70Δ::LEU2UCC2370 × UCC2374
UCC2719brad1::LEU2UCC2330b
UCC2720tel1Δ::LEU2UCC2402
UCC2769tel1Δ::LEU2UCC2338
UCC2770rad52::LEU2UCC2338
UCC2771rad1::LEU2UCC2332
UCC2814MATa-inc/MATα ade2::hisG/ade2::hisG his3Δ200/his3Δ200 leu2Δ0/leu2Δ0 lys2Δ1::GAL-HO/LYS2/lys2Δ1::GAL-HO/LYS2 met15Δ0/met15Δ0 trp1Δ63/trp1Δ63 ura3Δ0/ura3Δ0 can1Δ::ADE2/can1Δ::TRP1 mre11Δ::kanMX/MRE11 yku70Δ::LEU2/YKU70 rad50Δ::hph/RAD50UCC2499
UCC2842bsir2::LEU2 hmlΔ::hph hmrΔ::kanMXUCC2330b
UCC2843bsir3::LEU2 hmlΔ::hph hmrΔ::kanMXUCC2330b
UCC5843MATa-inc ade2::hisG his3Δ200 leu2Δ1 lys2Δ1::GAL-HO/LYS2 met15Δ0 trp1Δ63 ura3– 52Spore of BY4705a × DY3023c
  • a Brachmann et al. (1998).

  • b Strains used in assays described in the text and derived from UCC2330.

  • c Sweetser et al. (1994).