TABLE 1

Strains and plasmids

Strain/plasmidGenotypeReference
Strains
FSY157MATα ura3-52 leu2-3, 112 his3Δ200 lys2-801 tub1Δ::HIS3 tub3Δ::TRP1 plus pRB634Schatz et al. (1988)
FSY160MATα ura3-52 leu2-3, 112 his3Δ200 lys2-801 tub1Δ::HIS3 tub3Δ::TRP1 plus pRB629Schatz et al. (1988)
FSY182MATα ura3-52 leu2-3, 112 his3Δ200 lys2-801 tub1Δ::HIS3 tub3Δ::TRP1 plus pRB539Schatz et al. (1988)
FSY185a/α ura3-52/ura3-52 leu2-3,112/leu2-3,112 his3Δ200/his3Δ200 lys2-801/lys2-801 ade2/ADE2Weinstein and Solomon (1990)
JK418MATa leu2-3, 112 lys2 ura3 trp1 his3 ndc10-1 (W303)Goh and Kilmartin (1993)
KCY576a/α ura3-52/ura3-52 leu2-3,112/leu2-3,112 his3Δ200/his3Δ200 lys2-801/lys2-801 ade2/ADE2 BUB3/bub3::LEU2This study
KCY927FSY160 plus pRC4This study
KCY929FSY160 plus pKH502This study
KCY931FSY160 plus pKH130This study
KCY933FSY160 plus pKF44This study
KCY935FSY160 plus pMA1183This study
KCY936FSY160 plus pMA1145This study
KCY1087MATa ura3-2 leu2-3,112 lys2-801 his3Δ200 tub1::hisg tub3::hisg plus pKC43This study
KCY1168KCY1087 plus pRS315This study
KCY1170KCY1087 plus pTR168This study
KCY1295KCY1087 plus pTR170This study
KCY1876FSY160 plus pCT3This study
KCY1955FSY160 plus pDK7This study
KCY1956FSY160 plus pE58This study
KCY2010MATα tub1::HIS3 TUB3 trp1-1 lys2-801 leu2-3,112 ura3-52 his3Δ200 plus pRB629This study
KCY2011MATa tub1::HIS3 TUB3 trp1-1 lys2-801 leu2-3,112 ura3-52 his3Δ200 plus pRB629This study
KCY2013KCY2010 plus pCT3This study
KCY2014KCY2010 plus pDK7This study
KCY2015KCY2010 plus pE58This study
KCY2016KCY2010 plus pKF44This study
KCY2017KCY2010 plus pMA1183This study
KCY2018KCY2010 plus pMA1145This study
KCY2019KCY2010 plus pKH130This study
KCY2020KCY2010 plus pRC4This study
KCY2021KCY2010 plus pKH502This study
KCY2024MATa tub1::HIS3 tub3::KANR trp1-1 lys2-801 leu2-3,112 ura3-52 his3Δ200 plus pRB629This study
KCY2042MATa ura3-52 leu2-3,112 lys2-801 his3Δ200 trp1-1 tub1::HIS3 TUB3 mad2::KANR plus pRB629This study
KCY2048FSY160 plus pC2This study
KCY2070KCY2042 plus pCT3This study
KCY2071KCY2042 plus pKH130This study
KCY2072KCY2042 plus pRC4This study
KCY2073KCY2042 plus pKH502This study
KCY2074KCY2042 plus pE58This study
KCY2075KCY2042 plus pKF44This study
KCY2076KCY2042 plus pMA1138This study
KCY2077KCY2042 plus pMA1145This study
KCY2080KCY2042 plus pC2This study
KCY2085MATα ura3-52 leu2-3,112 his3Δ200 lys2-801 trp1-1 tub1::HIS3, tub3::KANR plus pRB629This study
KCY2098MATα ade2-101 ura3-52 leu2-3,112 his3Δ200 lys2-801 tub1::HIS3 tub3::KANR CFIII (CEN3.L.Y YPH278) URA3 SUP11 plus pRB629This study
KCY2103KCY2085 plus pDK7This study
KCY2155KCY2098 plus pRS317This study
KCY2156KCY2098 plus pKC52This study
KCY2157KCY2098 plus pKC74This study
KCY2171MATα ura3-52 his3Δ200 leu2-3,112 trp1-1 tub1::HIS3 tub3::KANR ndc10-1 plus pDK7 and pRB629This study
KCY2216MATα ura3-52 his3Δ200 leu2-3,112 trp1-1 tub1::HIS3 tub3::KANR dam1-1 plus pDK7 and pRB629This study
KCY2247MATa ura3-52 his3Δ200 leu2-3,112 trp1-1 tub1::HIS3 tub3::KANR ndc80-1 plus pDK7 and pRB629This study
KCY2202MATα ura3-52 his3Δ200 leu2-3,112 trp1-1 lys2-801 tub1::HIS3 bub3::LEU2 plus pKC43This study
KCY2258MATa ura3-52 his3Δ200 leu2-3,112 trp1-1 tub1::HIS3 tub3::KANR nuf2-61 plus pDK7 and pRB629This study
KCY2262MATa ura3-52 his3Δ200 leu2-3,112 trp1-1 lys2-801 tub1::HIS3 bub1::KANR plus pRB629This study
KCY2350KCY2202 plus pCT3This study
KCY2352KCY2202 plus pE58This study
KCY2354KCY2202 plus pKIF44This study
KCY2356KCY2202 plus pMA1183This study
KCY2358KCY2202 plus pMA1145This study
KCY2360KCY2202 plus pKH130This study
KCY2362KCY2202 plus pRC4This study
KCY2364KCY2202 plus pKH502This study
KCY2366KCY2262 plus pCT3This study
KCY2368KCY2262 plus pE58This study
KCY2370KCY2262 plus pKF44This study
KCY2372KCY2262 plus pMA1183This study
KCY2374KCY2262 plus pMA1145This study
KCY2376KCY2262 plus pKH130This study
KCY2378KCY2262 plus pRC4This study
KCY2380KCY2262 plus pKH502This study
KCY2397KCY2098 plus pKC44
MJYM14-9b (889)MATa his3Δ200 leu2-3,112 ura3-52 trp1Δ1 dam1-1Jones et al. (1999)
ndc80-1MATa ura3-52 his3Δ200 leu2-3,112 trp1-1 lys2-801 ndc80-1 (W303)Wigge et al. (1998)
PSY455MATa leu2 ura3 trp1 nuf2-61Osborne et al. (1994)
SKY294MATa tub1::HIS3 tub3::TPP1 mad2::KANR plus pDK7 and pRB629This study
YPH278MATα ade2-101 leu2-1 ura3-52 his3-200 lys2-801 CFIII (CEN3.L.Y YPH278) URA3 SUP11Spencer et al. (1990)
Plasmids
pAS53tub3::hisg-UPA3-hisgAbruzzi et al. (2002)
pC2BUB3-UPA3-2¼Guénette et al. (1995)
pCT3UPA3-CENGift of C. Thompson and R. Young
pDK7TUB1-UPA3-CENKirkpatrick and Solomon (1994)
pE58MPS1-UPA3-CENSchutz and Winey (1998)
pFA6-kanMX6KANR for constructing PCR knockoutsWach et al. (1997)
pKC43tub1-729-LYS2-CENThis study
pKC44TUB1-LYS2-CENThis study
pKC52BUB3-LYS2-CENThis study
pKC74BUB3-LYS2-2μThis study
pKF44BUB1-UPA3-CENFarr and Hoyt (1998)
pKH130MAD1-UPA3-CENHardwick and Murray (1995)
pKH502MAD3-UPA3-CENHardwick et al. (2000)
pKS198-dMAD2mad2::KANR deletion plasmidGift from K. Simons and P. Sorger
pLV61tub1::hisg-UPA3-hisgFleming et al. (2000)
pTR27bub3::LEU2Hoyt et al. (1991)
pTR168BUB1-LEU2-CENRoberts et al. (1994)
pTR170bub1K733R-LEU2-CENRoberts et al. (1994)
pMA1183BUB2-UPA3-CENGift from A. Hoyt
pMA1145BUB3-UPA3-CENRoberts et al. (1994)
pRB624tub1-724-LEU2-CENSchatz et al. (1988)
pRB539TUB1-LEU2-CENSchatz et al. (1988)
pRB629tub1-729-LEU2-CENSchatz et al. (1988)
pRC4MAD2-UPA3-CENChen et al. (1999)
pRS315LEU2-CENSikorski and Hieter (1989)
pRS317LYS2-CENSikorski and Hieter (1989)
YEp426LYS2-2μMa et al. (1987)