TABLE 6

Body length measurements of double mutants

Genotypea
(mm):
Wild typeaba + b-
wild typeb
a; ba × b; Pc
N2dbl-1(nk3)lon-3 (sp23)dbl-1(nk3) lon-3(sp23)
1.23 (±0.005)0.90 (±0.009)1.46 (±0.007)1.120.97 (±0.0009)<0.0001
N2dbl-1(nk3)lon-3 (e2175)dbl-1(nk3) lon-3(e2175)
1.23 (±0.005)0.90 (±0.009)1.34 (±0.008)1.001.03 (±0.007)0.06
N2sma-2 (e502)lon-3 (e2175)sma-2(502); lon-3(e2175)
1.23 (±0.001)0.61 (±0.001)1.56(±0.01)0.940.76 (±0.015)<0.0001
N2daf-4 (m63)lon-3 (e2175)daf-4(m63); lon-3(e2175)
1.23 (±0.001)0.73(±0.009)1.56(±0.01)1.031.16 (±0.015)<0.0001
N2dbl-1(nk3)lon-1 (e185)lon-1(e185); dbl-1(nk3)
1.23 (±0.06)0.90 (±0.08)1.48 (±0.09)1.181.17 (±0.007)0.09
1.26 (±0.005)0.85 (±0.10)1.59 (±0.013)1.171.13 (±0.011)0.03
1.29 (±0.009)II0.84 (±0.011)1.63 (±0.018)1.171.18 (±0.010)0.7 [0.3]
N2sma-2 (e502)lon-1 (e185)lon-1(e185); sma-2(e502)
1.23 (±0.001)0.61 (±0.001)1.57 (±0.010)0.940.93 (±0.012)0.3
1.26 (±0.005)0.64 (±0.007)1.57 (±0.014)0.950.96 (±0.008)0.8 [0.6]
N2daf-4 (m63)lon-1 (e185)lon-1(e185); daf-4(m63)
1.23 (±0.001)0.73 (±0.009)1.57 (±0.010)1.061.16 (±0.015)<0.0001
1.26 (±0.005)0.78 (±0.006)1.57 (±0.014)1.091.17 (±0.008)0.0001
[<0.0001]
N2lon-1 (e185)lon-3(sp23)lon-1(e185); lon-3(sp23)
1.27 (±0.005)1.59 (±0.013)1.62 (±0.10)1.941.94 (±0.16)0.10
1.29 (±0.005)d1.63 (±0.019)d1.62 (±0.02)d1.962.06 (±0.025)d0.002 [0.02]
N2lon-1 (e185)lon-3(e2175)lon-1(e185); lon-3(e2175)
1.25 (±0.005)1.67 (±0.010)1.61 (±0.01)2.041.99 (±0.018)0.03
1.31 (±0.006)d1.64 (±0.008)d1.55 (±0.06)d1.881.78 (±0.009)d<0.0001
[0.001]
N2sqt-1(sc103)lon-3 (sp23)sqt-1(sc103); lon-3(sp23)
1.43 (±0.10)1.29 (±0.011)1.87 (±0.009)1.721.57 (±0.007)<0.0001
N2sqt-1(sc103)svEx57sqt-1(sc103); svEx57
1.34 (±0.008)1.19 (±0.008)1.21 (±0.016)1.061.31 (±0.007)<0.0001
N2sqt-1(sc103)dbl-1 (ctls40)sqt-1(sc103); dbl-1(ctls40)
1.34 (±0.01)1.26 (±0.009)1.53 (±0.01)1.451.22 (±0.008)<0.0001
N2rol-6(e187n1268)lon-3 (sp23)rol-6(e187n1268); lon-3(sp23)
1.37 (±0.007)1.17 (±0.007)1.69 (±0.007)1.491.34 (±0.005)<0.0001
  • Worms in each row represent one set of measurements in which worms of the different genotypes were cultured under as identical as possible growth conditions. Small variations are seen in the length measurements for a given genotype between different experiments. These variations may result from variations in the osmolarity or consistency of the plates used. We have noticed that worms grown on drier plates (or on plates with a higher agar concentration) tend to be slightly longer than worms of the same genotype grown on wetter plates (or plates with lower agar content).

  • a Means (±SE). N = 27-70; all worms measured at 96 hr posthatching unless stated otherwise.

  • b a + b-wild type is the estimated additive value. It represents the phenotypic value of the double homozygous mutant a;b in the absence of any interaction between a and b.

  • c a × b; P is the probability of the interaction between a and b being due to chance alone. It is estimated as the interaction term of a two-factor, fixed effects analysis of variance. Combined probabilities of several experiments given in brackets.

  • d Measured at 120 hr posthatching.