TABLE 1

Classification and phenotypic characterization of WS- mutants

GroupNo. in groupaColony
morphologyb
Microcosm
diversificationc
Colonization
of air-broth interfaced
I6SMSMWSFS-
II14SMSMFS-
III3SM°SM-
IV2SM°°SM-
V1SMSM±
VI1SM°SM±
  • a Number of unique mutants in group: Group I, WS-4, -7, -11, -16, -29, and -40; Group II, WS-1, -2, -13, -15, -20, -21, -22, -25, -27, -28, -30, -33, -34, and -35; Group III, WS-6, -9, and -18; Group IV, WS-12 and -36; Group V, WS-39; Group VI, WS-5.

  • b Colony morphology on KB agar: SM, smooth colonies; SM°, develops WS-like characteristics after long incubations on KB agar; SM°°, SM on KB but WS-like on LB agar; WS, wrinkly spreader-like colonies.

  • c Colony morphs that arise after selection (7 days) in spatially structured microcosms; FS, fuzzy spreader-like colonies; —, none detected.

  • d Evidence of niche specialization as determined by mat formation in spatially structured microcosms; -, no mat, all growth within the broth phase; ±, thin, easily collapsed mat, most growth in broth phase.