TABLE 4

Effect of presynaptic machinery mutants on Pir

Halothane EC50a
StrainWithoutWithFold changeMutation
N20.42 ± 0.030.95 ± 0.022.3
snb-1(md247)0.11 ± 0.04b0.19 ± 0.061.7*Rf in VAMP
ric-4(md1088)0.22 ± 0.04b0.66 ± 0.083.0*Rf in SNAP-25
unc-64(js21)0.17 ± 0.03b0.45 ± 0.052.6*Rf in syntaxin
unc-64(md130)1.06 ± 0.11c2.08 ± 0.232.0*Rf in syntaxin
goa-1(n363);unc-64(md130)1.33 ± 0.06c1.40 ± 0.061.1goa-1(null);unc-64(rf)
  • Rf, reduction of function. *Significant Pir, P < 0.05.

  • a All EC50’s were calculated from at least three independent experiments with at least six halothane concentrations/experiment.

  • b Significantly hypersensitive compared to N2 under “without pheromone” condition at P < 0.05.

  • c Significantly resistant compared to N2 under “without pheromone” condition at P < 0.05.