TABLE 3

Effect of neurotransmitter/receptor mutations on Pir

Halothane EC50a
StrainWithoutWithFold changeMutation
N20.42 ± 0.030.95 ± 0.022.3*None
unc-49(e382)0.37 ± 0.050.75 ± 0.132.0*Rf in GABA receptor
unc-25(e156)0.30 ± 0.020.52 ± 0.071.7*Null in GAD
cat-2(e1112)0.33 ± 0.061.01 ± 0.053.1*Rf in dopamine biosynthesis
bas-1(ad446)0.56 ± 0.131.15 ± 0.202.1*Rf in serotonin biosynthesis
cat-4(e1141)0.38 ± 0.110.75 ± 0.142.0*Rf in dopamine/serotonin biosynthesis
glr-1(n2461)0.64 ± 0.041.08 ± 0.131.7*Rf in glutamate receptor
  • Rf, reduction of function; Gf, gain of function; GAD, glutamic acid decarboxylase. *Significant Pir, P < 0.05.

  • a All EC50’s were calculated from at least three independent experiments with at least six halothane concentrations/experiment.