TABLE 2

Effect of Goα pathway mutations on Pir

Halothane EC50a
GenotypeWithout pheromoneWith pheromoneFold changeMutation
N20.42 ± 0.030.95 ± 0.022.3*None
goa-1(sy192)1.07 ± 0.04b0.80 ± 0.080.7Dominant negative in Goα
goa-1(n363)0.43 ± 0.030.52 ± 0.051.2Null
goa-1(n1134)0.47 ± 0.040.43 ± 0.040.9Null
goa-1(pk62)0.45 ± 0.050.48 ± 0.011.1Rf
egl-10(nIs51)1.06 ± 0.04b0.88 ± 0.040.8Gf in RGS for Goα
egl-10(n480)0.52 ± 0.010.47 ± 0.040.9Rf
egl-10(md176)0.45 ± 0.120.29 ± 0.050.6Rf
sag-1(sy428)0.28 ± 0.020.24 ± 0.020.9Lf in DGK-1
eat-16(sy438)0.77 ± 0.04b0.85 ± 0.081.1Lf in RGS for Gqα
  • Rf, reduction of function; Gf, gain of function; Lf, loss of function. *Significant Pir, P < 0.05.

  • a All EC50’s were calculated from at least three independent experiments with at least six halothane concentrations/experiment.

  • b Significantly resistant compared to N2 without pheromone condition.