TABLE 1

Effect of mutations in dauer pathway genes on pheromone-induced halothane resistance

Halothane EC50
GenotypeWithout
pheromone
With
pheromone
Fold
changea
N2 (wild type)0.42 ± 0.030.95 ± 0.022.3b
daf-22(m130)0.48 ± 0.030.70 ± 0.051.5b
daf-11(m47)0.29 ± 0.060.30 ± 0.071.0
daf-21(p673)0.64 ± 0.120.62 ± 0.071.0
osm-1(p808)0.56 ± 0.07c0.78 ± 0.031.4
osm-3(p802)0.50 ± 0.030.52 ± 0.031.0
osm-5(p813)0.50 ± 0.010.50 ± 0.011.0
osm-6(p811)0.33 ± 0.010.33 ± 0.011.0
che-2(e1033)0.42 ± 0.030.31 ± 0.010.74
che-3(e1124)0.52 ± 0.040.42 ± 0.030.81
che-11(e1810)0.35 ± 0.010.37 ± 0.011.1
che-13(e1805)0.42 ± 0.010.31 ± 0.010.74
daf-10(e1387)0.39 ± 0.070.38 ± 0.041.0
daf-1(m40)0.21 ± 0.02d0.37 ± 0.061.8b
daf-4(e1364)0.38 ± 0.060.95 ± 0.172.5b
daf-7(e1372)0.20 ± 0.08d0.36 ± 0.071.8b
daf-8(e1393)0.13 ± 0.07d0.38 ± 0.012.9b
daf-3(e1376)0.63 ± 0.06c0.92 ± 0.161.5b
daf-5(e1386)0.55 ± 0.06c0.80 ± 0.111.5b
daf-12(m20)0.59 ± 0.04c0.91 ± 0.061.5b
  • a Ratio of the halothane EC50 with N2 pheromone to the halothane EC50 without pheromone.

  • b A significant increase in halothane EC50 with pheromone vs. without (P < 0.05); statistical significance determined by simultaneous curve fitting (Waud 1972; DeLean et al. 1978).

  • c Significantly resistant compared to N2 without pheromone condition (P < 0.05).

  • d Significantly hypersensitive compared to N2 without pheromone condition (P < 0.05).