TABLE 2

High levels of salicylic acid accumulation in cim mutants are correlated to PR-1 gene expression levels

MutantTotal SA (ng/mg FW)PR-1a
6E (wild type)296 ± 251.0
6E + Erysiphe2030 ± 89025b
cim51958 ± 83511.4
cim61657 ± 43617.5
cim7899 ± 162.9
cim8294 ± 211.7
cim94154 ± 21138.2
cim102256 ± 22311.8
cim111500 ± 7812.6
cim121350 ± 26710.5
cim133415 ± 3317.3
cim142190 ± 49124.7
6E + BTHNA11.0
  • PR-1 gene expression levels were quantified using a phosphorimager and normalizing for loading differences with α-tubulin. The 6E line showed consistently wild-type levels of PR-1 gene expression. SA, salicylic acid; FW, fresh weight.

  • a PR-1 expression relative to wild type.

  • b PR-1 expression in Peronospora parasitica pv Noco2-infected tissue (8 days after inoculation).