TABLE 9

Chitosan synthesis in ADY3/ADY3 and ady3Δ/ady3Δ cells

StrainRelevant genotypeNo. of chitosan+ prospores/cell:Distribution of cell types (%)
01234
AN120ADY3/ADY358.61.42.36.434.3
AN246ady3Δ/ady3Δ92.45.11.70.30.5
  • Cells were cultured for 8.5 hr in 2% potassium acetate and fixed. Aliquots from each sample were stained separately with either DAPI or Calcofluor white. A total of 200 cells were analyzed to assay meiosis, and 1000 Calcofluor white-stained cells were analyzed to assay chitosan synthesis. Totals of 53.5% of AN120 cells and 72% of AN246 cells in these samples had completed meiosis.