TABLE 8

β-1,3-glucan synthesis in ANY3/ADY3 and ady3Δ/ady3Δ cells

StrainRelevant genotypeNo. of β-1,3-glucan+ prospores/cell:Distribution of cell types (%)
01234
AN120ADY3/ADY36211234
AN246ady3Δ/ady3Δ76.5153.52.52.5
  • Cells were cultured for 8 hr in 2% potassium acetate, fixed, and processed for immunofluorescence microscopy. A total of 200 cells that had completed meiosis and contained four prospore membranes as judged by staining with DAPI and FITC-ConA were analyzed.