TABLE 4

Two-hybrid interactions with LexA-Ady3p

GAD fusionInteraction
None
Cnm67p386-580++
Gip1p161-573
Mpc70p1-609+++
Nud1p405-852+++
Spc42p1-363+
  • A LexA-Ady3p fusion was tested for interactions with fusions of the Gal4 activation domain (GAD) to residues of indicated proteins using a β-galactosidase filter lift assay as described in materials and methods. Three independent transformants for each combination of constructs were analyzed. None of GAD fusions used here interacted with LexA alone. The following LexA fusions were used as positive controls for interactions with GAD fusions: Glc7p1-312 for Gip1p161-573; Nud1p405-852 for Mpc70p1-609 and Nud1p405-852; and Cnm67p386-580 for Spc42p1-363.