TABLE 2

Molecular characterization of cDNA clones obtained by cDNA-AFLP

cDNA AFLP patterna
Clone identificationSlSlAmAmSlSlAmAmSequence similarityb
RAIF1–3+None
RAIF4–6+ORF (putative proteins)
RAIF7+Rice EST
RAIF8+Ae. speltoides genome-specific DNA
RAIF9+Wheat Wis 2-1A retrotransposon
RAIF10–11+None
RAIF12–16+ORF (putative proteins)
RAIF17–18+Wheat DNA for ORF flanked by ARS
RAIF19–23+Wheat EST
RAIF24–26+Rye 26S rRNA
RAIF27+Gene cluster 21-kb wheat chloroplast DNA
RAIF28+Wheat rubisco-large subunit
RAIF29+Wheat mitochondrion rrn26 gene for rRNA
RAIF30+Rice alpha 2 subunit of 20S proteasome
RAIF31+Agropyron cristatum-RAPD marker
RAIF32+Wheat acetyl-coenzyme A carboxylase
RAIF33+Wheat retrotransposon Tar1
RAIF34+Barley NBS/LRR resistance gene RPM1
RAIF35+Human cell cycle regulatory protein p95
RAIF36++ORF (putative proteins)
RAIF37++Wheat EST
RAIF38++Arabidopsis thaliana putative retroelement
RAIF39++Succinyl-diaminopimelate desuccinylase
RAIF40++A. thaliana Ring-h2 finger protein
RAIF41–42++A. thaliana acylaminoacyl-peptidase
RAIF43–44+None
RAIF45+Maize retrotransposon (gag)
RAIF46–48+Wheat Wis2-1A retrotransposon
  • RAPD, randomly amplified polymorphic DNA.

  • a +, transcript present; −, transcript absent.

  • b The search was done using the BLAST package. Similarity was considered as significant for E-values < e−10.