cDNA-AFLP analysis in an allotetraploid and its diploid progenitors
| Expression alteration in allopolyploidb | ||||
|---|---|---|---|---|
| Tissue | Total no. of transcriptsa | Transcript absent in allopolyploid (%) | New transcript in allopolyploid (%) | Total alterations (%) |
| Leaves | 1872 | 29 (1.6) | 10 (0.5) | 39 (2.1) |
| Root tips | 1200 | 19 (1.6) | 2 (0.2) | 21 (1.8) |
| Total | 3072 | 48 (1.6) | 12 (0.4) | 60 (2.0) |
↵a Each cDNA-AFLP band was considered as a different transcript. The 1872 leaf transcripts are derived from 37 pairs of selective primers and the 1200 root-tip transcripts are from 25 pairs of selective primers. Monomorphic transcripts (similar in both parents) were scored only once.
↵b Only qualitative alterations were scored, namely, disappearance of parental transcripts in the allotetraploid or appearance of a new transcript in the allotetraploid. The percentage of altered bands is out of the total number of transcripts.