TABLE 7

Tetrad analysis of mlh1ts/mlh1Δ diploids sporulated and germinated at 30°

Marker at ARG4: MMR genotype: Diploid:ARG4/arg4-Bglll
MLH1 5mlh1Δ 6mlh1-T113A 7mlh1-I147T 8mlh1-F228S 9mlh1-I296S 10mlh1-I296S at 26° 10
Tetrads scored511712474534540545352
Spore viability (%)90688184817995
ARG4 aberrants (%)1.65.62.31.3 5.25.10.9
PMS/ARG4 aberrants0/813/400/110/79/286/280/3
URA3-TRP1 genetic distance (cM)5.12.94.94.45.84.66.0
PD:TT:NPDa444:50:0630:39:0418:45:0474:46:0461:47:2473:48:0300:41:0
ADE2-HIS3 genetic distance (cM)39.023.037.537.432.334.238.1
PD:TT:NPDa187:309:15409:298:5178:283:12175:268:12251:277:12232:300:12129:214:9
  • The indicated diploid strains (Table 1) were analyzed as described in Table 5.

  • a Only tetrads that contained Mendelian segregations for the markers located at the indicated intervals were used to measure genetic distances.