TABLE 6

Canavanine patch assay of mlh1ts strains grown at 30°

StrainGenotypeMedian no. of canavanine-resistant papillations for each experimentAverage fold increase in canavanine papillation relative to MLH1
EAY777MLH14, 2, 15, 7, 7, 6, 51
EAY806mlh1Δ::hisG125, 101, 123, 144, 13919.2
EAY831mlh1-T113A4, 6, 4, 3, 13, 111.0
EAY833mlh1-I147T8, 6, 8, 6, 5, 51.0
EAY829mlh1-F228S3, 6, 9, 7, 6, 15, 8, 121.3
EAY836mlh1-I296S9, 7, 6, 4, 5, 30.9
  • Single colonies of the indicated strains were grown on YPD for 3 days at 30°, after which 11 independent colonies (2 mm in diameter) were patched onto canavanine plates. In each experiment, the median number of canavanine papillations scored after a 3-day incubation at 30° is presented.