TABLE 2

lys2-BglII reversion rates in strains containing conditional mlh1 mutations

26°35°
Relevant genotypeRate relative to wtAverageRate relative to wtAverage
Wild type0.94, 1.11.00.91, 1.3, 0.821.0
mlh1Δ39, 393950, 39, 3642
exo1Δa3.5, 2.83.13.5, 3.43.5
mlh1-T113A0.72, 0.880.871, 2448
mlh1-I147T0.69, 2.11.454, 3344
mlh1-F228S1.2, 1.71.580, 3055
mlh1-I296S1.0, 2.21.667, 3953
  • EAY652 (mlh1Δ, lys2-BglII), was transformed with pEAA109 (MLH1 ARSH4 CEN6)-derived plasmids carrying the indicated mlh1 alleles. Reversion rates per generation were determined as described in materials and methods and are reported relative to EAY652 transformed with pEAA109 at the respective temperature (1.6 × 10−8 at 26°, 2.2 × 10−8 at 35°). The reversion rate of each independent experiment is presented (median of 6–11 cultures; Lea and Coulson 1949). wt, wild type.

  • a Data from Sokolsky and Alani (2000).