TABLE 1

Diploid strains used in the meiotic analysis of the mlh1ts mutations

Diploid no.Strain
1(EAY506) MATa yhr020W::URA3 arg4-RV yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 his3Δ1 ade2
(EAY512) MATα YHR020W ARG4 YHR017W ura3::YHR020W leu2-3,112 trp1-289 HIS3 ADE2
2(EAY492) MATa yhr020W::URA3 ARG4 yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 his3Δ ade2 cyhr
(EAY502) MATα YHR020W arg4-BglII YHR017W ura3::YHR020W leu2-3,112 trp1-289 HIS3 ADE2 cyhs
3(EAY629) MATa yhr020W::URA3 arg4-RV yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 his3Δ1 ade2 mlh1Δ::LEU2
(EAY630) MATα YHR020W ARG4 YHR017W ura3::YHR020W leu2-3,112 trp1-289 HIS3 ADE2 mlh1Δ::LEU2
4(EAY627) MATa yhr020W::URA3 ARG4 yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 his3Δ1 ade2 cyhr mlh1Δ::LEU2
(EAY628) MATα YHR020W arg4-BglII YHR017W ura3::YHR020W leu2-3,112 trp1-289 HIS3 ADE2 cyhs mlh1Δ::LEU2
5(EAY778) MATa yhr020W::URA3 arg4-BglII YHR017W ura3::YHR020W leu2-3,112 trp1-289 his3Δ1 ADE2 cyhr MLH1
(EAY806) MATa YHR020W ARG4 yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 HIS3 ade2 cyhs mlh1Δ::hisG
6(EAY805) MATa yhr020W::URA3 arg4-BglII YHR017W ura3::YHR020W leu2-3,112 trp1-289 his3Δ1 ADE2 cyhr mlh1Δ::hisG
(EAY806) MATa YHR020W ARG4 yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 HIS3 ade2 cyhs mlh1Δ::hisG
7(EAY805) MATα yhr020W::URA3 arg4-BglII YHR017W ura3::YHR020W leu2-3,112 trp1-289 his3Δ1 ADE2 cyhr mlh1Δ::hisG
(EAY831) MATa YHR020W ARG4 yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 HIS3 ade2 cyhs mlh1-T113A
8(EAY805) MATα yhr020W::URA3 arg4-BglII YHR017W ura3::YHR020W leu2-3,112 trp1-289 his3Δ1 ADE2 cyhr mlh1Δ::hisG
(EAY833) MATa YHR020W ARG4 yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 HIS3 ade2 cyhs mlh1-I147T
9(EAY805) MATα yhr020W::URA3 arg4-BglII YHR017W ura3::YHR020W leu2-3,112 trp1-289 his3Δ1 ADE2 cyhr mlh1Δ::hisG
(EAY829) MATa YHR020W ARG4 yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 HIS3 ade2 cyhs mlh1-F228S
10(EAY805) MATα yhr020W::URA3 arg4-BglII YHR017W ura3::YHR020W leu2-3,112 trp1-289 his3Δ1 ADE2 cyhr mlh1Δ::hisG
(EAY836) MATa YHR020W ARG4 yhr017W::TRP1 ura3::YHR020W leu2-3,112 trp1-289 HIS3 ade2 cyhs mlh1-I296S
  • The above strains were from the MGD strain background (Rocco et al. 1992; materials and methods). The arg4-Bg111 marker contains a 4-bp insertion at +1274 in ARRG4, and the arg4-RV marker contains a 2-bp deletion at +260 in ARG4. The location of the mutation in the various ARG4 markers is shown with respect to the first nucleotide in the initiating ATG.