TABLE 2

Primers and oligonucleotide probes

DesignationLengthSequence (5′ → 3′)
PCR primers
16S + 122CCGGTCTGAA CTCAGATCAA GT
16S − 129CTGCTCAATG ATTTTTTAAA TTGCTGTGG
16S + 221CGCTGTTATC CCTAGAGTAT T
16S − 222TTGGGCAAGA AGACCCTATG AA
OA-ext (+)23AAAAAATATT TATTGGGAAT AGG
OA-ext (−)29GTTTTTTTAC TGTTTACAGT AATTGTTAA
OA-int (+)16GGAGTACTTG AAGGCG
OA-int (−)18GCTTAAAGTA ACAGTTCC
OC6 (+)24AAAGAATACA TTAAGTGCGA TATT
Fluo-OC6 (+)24Fluorescein-AAAGAATACA TTAAGTGCGA TATT
OC602 (−)22GGGCTTGTAA GCTCTTTAAC TG
OC623 (−)24TTAAGGTTTT TTTTGGACTT TCTGC
RLB probes
OC-ALL26AGATTAGGCC CTTTAACAGA CTCATC
OC-A22ATTGTGATTA TTTTCGGTAT CC
OC-B16CTCGTTGCGA TTTGCT
OC-CNo specific probes (a recombinant between B, I, and E)
OC-D36ATGATTATTT AGAGTGCCTA AAGCATTGTT TTGATC
OC-E39TGTGTTTTTA CTCTGATTGG CCTCTAAACC ATTATTGCC
OC-F27CGCCTGAACG CCTAAACCAT TTGCATC
OC-G16GGTGTTGTGA TTCGCA
OC-H30GCCCCCATCG TCACCCAAAG TGCCATTTTG
OC-I23TTTGAAATTA AATATGCTCC TGA
OC-J16TCCGTTTTGA CCCACT
OC-K33CCCCGCTTCG CTACCTAAAC CAGCATTTTG TTG
OC-L27ATCGCTACCT AAAGTACCAC CTGCTTC
OC-M31ACCGGCATTT AAACCATTTT GGGCTATCAA A
OC-N30GTTTTGCACA TCATCTAAAC CATTATTATT
OC-O22TTGGTTAACT AAGCCATTTG CC
OC-T18ATGGCCTGCA TCGACACT
OC-U19CTGCCCTTGC AAGTCCTGT