TABLE 4

Amino acid substitutions in naturally occurring extended-spectrum TEM alleles

Amino acid position
Enzyme2139425192104153164182237238240244265268
TEM-1LQALGEHRMAGERTS
TEM-2aKKS
TEM-3KKS
TEM-5STK
TEM-6KH
TEM-8KKSS
TEM-9FKSM
TEM-10SK
TEM-11KH
TEM-12S
TEM-15KS
TEM-17K
TEM-20TS
TEM-21KKRS
TEM-22KKGS
TEM-24KKSTK
TEM-25FSM
TEM-26KS
TEM-27HKM
TEM-28HK
TEM-29H
TEM-42KVSKM
TEM-43KHT
TEM-47SKM
TEM-48FSKM
TEM-49FSKMG
TEM-53FS
TEM-54L
TEM-60KPKS
TEM-66KDKS
TEM-72TSK
No. of times the mutation has arisen3111171H5 S73G1 T256121
  • Data are from http://www.lahey.org/studies/webt.htm. The number of times each mutation has arisen independently is based on the phylogeny shown in Figure 1.

  • a TEM-2 is not an extended-spectrum allele but is shown because the Q39K substitution that is found in nine extended-spectrum alleles arose in TEM-2.