TABLE 3

Mutations recovered from in vitro evolved TEM alleles

Amino acid substitutionb
DNA siteaMutation1234567a7b89
11A>GQ6R
45G>As
105A>Gss
199C>GA42G
220C>Ts
304G>A(E104K)(E104K)(E104K)(E104K)(E104K)(E104K)(E104K)
484C>A(R164S)(R164S)R164Nc(R164S)
485G>A(R164H)R164Nc
496G>AE168K
498G>As
511A>GI173VI173VI173V
512T>GI173T
539T>C(M182T)(M182T)
546A>Gs
562T>Cs
571C>Ts
660C>Ts
697T>As
703G>TA237SA237S
706G>A(G238S)(G238S)(G238S)(G238S)(G238S)(G238S)
709G>A(E240K)(E240K)(E240K)
748G>CD254H
749A>GD254G
756G>As
782C>T(T265M)(T265M)
784A>GT266A
787G>AG267R
825C>As
  • Substitutions that have arisen multiple times in nature are in parentheses. s, a silent mutation.

  • a Position in the coding sequence.

  • b Substitutions are given as the TEM-1 amino acid according to the IUPAC single-letter code, the position in the protein numbered according to Ambler et al. (1991), and the mutant amino acid. E104K means that the glutamate at position 104 of TEM-1 was replaced by lysine.

  • c The R164N mutation is the result of two base substitutions.