MICs of evolved TEM alleles
| Isolate no. | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Druga | Clinical MICb | pACSE3 | TEM1 | 1 | 2 | 3 | 4 | 5 | 6 | 7a | 7b | 8 | 9 |
| AMC | 32 | 2 | >4096 | >4096 | 4096 | 4096 | 4096 | 4096 | >4096 | >4096 | 4096 | >4096 | 4096 |
| PIP | 128 | 2 | >2048 | 2048 | 2048 | 2048 | 2048 | 2048 | 2048 | 4096 | 1024 | 2048 | 2048 |
| TZP | 128 | 1 | 256 | 16 | 16 | 64 | 32 | 128 | 32 | 64 | 32 | 32 | 32 |
| TIC | 128 | 4 | >4096 | >4096 | >4096 | >4096 | >4096 | >4096 | >4096 | >4096 | >4096 | 2048 | >4096 |
| TIM | 128 | 4 | 512 | 256 | 128 | 128 | 128 | 256 | 128 | 128 | 64 | 128 | 128 |
| CEF | 32 | 4 | 1024 | 512 | 512 | 1024 | 512 | 512 | 64 | 256 | 64 | 64 | 256 |
| CXM | 32 | 8 | 16 | 512 | 512 | 1024 | 1024 | 1024 | 32 | 128 | 64 | 32 | 512 |
| (CRO) | 64 | 0.063 | 0.25 | 512 | 128 | 64 | 128 | 64 | 32 | 32 | 16 | 32 | 64 |
| (CTX) | 64 | 0.125 | 0.125 | 512 | 256 | 512 | 256 | 512 | 32 | 32 | 16 | 32 | 32 |
| (CAZ) | 32 | 0.25 | 1 | 128 | 64 | 64 | 64 | 512 | 2048 | 2048 | 512 | 2048 | 2048 |
| FEP | 32 | 0.063 | 0.5 | 16 | 8 | 8 | 8 | 8 | 32 | 16 | 4 | 64 | 8 |
| (ATM) | 32 | 0.125 | 0.5 | 32 | 64 | 64 | 64 | 128 | 512 | 1024 | 128 | 1024 | 512 |
The MICs of the antibiotic in which the lines were first selected appear italic.
↵a Abbreviations for (β-lactams are the following: AMC, ampicillin; PIP, pipericillin; TZP, pipericillin and tazobactam (8:1); TIC, ticarcillin; TIM, ticarcillin and clavulanate; CEF, cephalothin; CXM, cefuroxime; CRO, ceftriaxone; CTX, cefotaxime; CAZ, ceftazadime; FEP, cefepime; ATM, aztreonam. The “extended-spectrum” drugs used for selection are in parentheses.
↵b The indicated MICs are the NCCLS breakpoints for resistance (National Committee for Clinical Laboratory Standards 1999).