TABLE 5

Nucleotide composition of 315 unique RescueMu insertion sites

Sequence 5′ of TSD
Nucleotide−10−9−8−7−6−5−4−3−2−1Exp.a
C12311811110413292103189**226**93110
G50*61777866726122**28**30**73
A567065574577754724 **5763
T866662767274765747*135**69
Sum χ213**3.61.41.79.66.85.598**190**88**0
TSD
Nucleotide123456789Exp.
C154**128150**103137*1268881*162**110
G8136**41**887758110**788373
A37**38**33**42*4883*7096**32**63
T43**113**91*825348*47*6038**69
Sum χ239**57**48**12**14**19**33**28**55**0
Sequence 3′ of TSD
Nucleotide+1+2+3+4+5+6+7+8+9+10Exp.
C78**81*47**114137*109109119109121110
G59161**135**7555758673697473
A113**41*674554776655685063
T6532**668169545468697069
Sum χ254**140**91**6.612**7.46.61.60.773.40
  • Composition analysis of 315 RescueMu insertion sites as defined by the 10 nucleotides 5′ of the 9-bp TSD, the 9-bp TSD, and the 10 nucleotides 3′ of the 9-bp TSD. Chi-square values were calculated for each individual nucleotide at all positions and the sum of those values is listed for each position in the table. The symbols * and ** indicate chi-square values or nucleotides frequencies with chi-square values that are significantly different from expected at the 95 and 99% confidence intervals, respectively. Underlined and boxed text indicate the nucleotides that occur at significantly higher-than-expected rates at the 95 and 99% confidence intervals, respectively, and therefore are consensus nucleotides at that position. Italics indicate nucleotides that are significantly underrepresented at a given position.

  • a Expected nucleotide compositions represent the number of times each nucleotide is expected to appear at each position based on the nucleotide composition of the 315 combined insertion sites.