TABLE 2

Nucleotide composition of 35 unique insertion sites

Sequence 5′ of TSD
Nucleotide−10−9−8−7−6−5−4−3−2−1Exp.a
C9111621*81691230**2**13
G145113*155872*1510
A7122*8813*12101*127.5
T57634166264.3
Sum χ22.87.45.410*4.59.8*5.12.636**15**
TSD
Nucleotide123456789Exp.
C1791914141264*23**13
G107111112111317*410
A51042*6912847.5
T39*18334644.3
Sum χ22.58.2*6.97.31.10.837.311*13**
Sequence 3′ of TSD
Nucleotide+1+2+3+4+5+6+7+8+9+10Exp.
C9102**1416151315131713
G1020**141510895101310
A15**1*14*0*68813*537.5
T14563452724.3
Sum χ211*16**16**11*1.40.830.288.2*2.55.9
  • A total of 35 g18-Mu insertion alleles from Table 1 have unique insertion sites as defined by the 10 nucleotides 5′ of the 9-bp TSD, the 9-bp TSD, and the 10 nucleotides 3′ of the 9-bp TSD. Chi-square values were calculated for each individual nucleotide at all positions and the sum of those values is listed for each position in the table. The symbols * and ** indicate chi-square values or nucleotides frequencies with chi-square values that are significantly different from expected at the 95 and 99% confidence intervals, respectively. Underlined and boxed text indicate the nucleotides that occur at significantly higher-than-expected rates at the 95 and 99% confidence intervals, respectively, and therefore are consensus nucleotides at that position. Italics indicate nucleotides that are significantly underrepresented at a given position.

  • a Expected nucleotide compositions represent the number of times each nucleotide is expected to appear at each position based on the nucleotide composition of the 35 combined insertion sites.