TABLE 3

Brood size of untreated RAD-51(+) and corresponding RAD-51-depleted worms

wt*mut-7nuc-1*him-3*spo-11*rad-5ced-3*
RAD-51++(+)a+++++
N F11435422811555610112843
Average no. of eggs224 ± 43138 ± 2687 ± 1386 ± 15136 ± 2268 ± 20212 ± 57115 ± 25203 ± 10153 ± 10127 ± 23117 ± 31177 ± 44138 ± 53
RAD-51/RAD-51+0.620.990.500.540.750.920.78
  • Analysis using z-statistics shows that in all the strains indicated with the asterisks untreated and rad-51-interfered populations are significantly different (PRAD51+=RAD51 ≤ 0.001). N F1, the number of F1 individuals whose progeny were scored.

  • a The data relative to the RNAi-resistant mut-7 worms treated with the same procedure used to induce RNAi in the other strains. Only in the rad-5 genetic background and in the RNAi-resistant control strain mut-7 are untreated and rad-51-interfered populations not significantly different.