TABLE 3

Efficiency of generation of mutant phenotypes

ControlpCAP59ipCAP59-noPpCAP59i-telpADE2ipCAP59/ADE2i
Dull<110<127<124.5
Pink<1<1<1<1725.5
  • Cells were transformed with the indicated plasmids as described in materials and methods, and at least 100 transformants for each plasmid were restreaked for single colonies and examined for phenotype. The percentage of transformants demonstrating inhibition of CAP59 expression (dull colonies) or ADE2 expression (pink colonies) is tabulated for each plasmid.