TABLE 1

Surface-targeted trf4 mutant yeast strains

StrainAlleleAmino acid changesPlasmidCPTa sensitivityViability in Δtrf5 background
CY1187TRF4NoneCB905NoViable
CY1181trf4-12K12A, K13A, K15ACB1084NoViable
CY1182trf4-21K21A, K23A, K24ACB1085NoViable
CY1183trf4-78D78A, D79A, D80ACB1086NoViable
CY1186trf4-101E101A, E102A, E104ACB1088NoViable
CY1184trf4-121D121A, D122ACB1087NoViable
CY1197trf4-131E131A, D132A, E133ACB1081NoSlow growth
CY1249trf4-140E140A, R141A, E142ACB1100NoSlow growth
CY1261trf4-145E145A, E147ACB1128NoViable
CY1193trf4-182E182A, K184A, D185ACB1089YesLethal
CY1194trf4-194E196A, E198A, R194A, E195ACB1090YesLethal
CY1251trf4-217D217A, D219ACB1093NoSlow growth
CY1625trf4-224G224A, S225ACB1354YesLethal
CY1262trf4-236D236A, D238ACB1129YesLethal
CY1252trf4-248K248A, E249A, R251ACB1091NoViable
CY1263trf4-262K262A, K263A, K264ACB1130NoViable
CY1295trf4-275K275A, R277ACB1135NoTemperature sensitive
CY1291trf4-282K282A, E285ACB1134YesLethal
CY1296trf4-309R309A, E310ACB1137NoLethal
CY1292trf4-332R332A, R333ACB1132NoTemperature sensitive
CY1200trf4-369K369A, D370ACB1099NoViable
CY1264trf4-378E378A, E381ACB1126YesLethal
CY1269trf4-390D390A, D391ACB1096NoViable
CY1248trf4-425D425A, D428A, E429ACB1121NoLethal
CY1195trf4-444K444A, K445ACB1094YesLethal
CY1265trf4-467K467A, D468A, R469ACB1131NoTemperature sensitive
CY1196trf4-486R486A, D487A, D490ACB1095NoLethal
CY1270trf4-491E491A, R492ACB1097YesLethal
CY1293trf4-502E502A, E504ACB1133NoLethal
CY1294trf4-508K508A, K509A, R510ACB1136YesLethal
CY1259trf4-521E521A, D522ACB1124NoViable
CY1267trf4-545K545A, K546A, K548ACB1122NoViable
CY1266trf4-554K554A, E556ACB1127NoViable
CY1268trf4-559K559A, R560ACB1123NoSlow growth
CY1199trf4-572E572A, D573A, D574ACB1098NoSlow growth
  • All strains are isogenic with CY1187 (MATa TRF4-HIS3 trf5::LEU2 his3-11,15 leu2-3,112 trp1-1 ura3-1 ade2-1 + pURA3.TRF5) with the exception of the trf4 allele. Plasmids used for integration of the trf4 mutants are listed.

  • a Camptothecin sensitivity in a TRF5 background. Strains that are CPT sensitive are at least 10-fold more sensitive to the drug than the wild-type strain.