TABLE 1

Genes identified in the mating-type loci

Gene nameMessage size (kb)When expressedB valuea% GCbComments
Genes in both mating types, T domain
Pr21.9All stages
Nic7NDND, presumed vegetative0.61c69cNicotinamide-requiring mutant; possible quinolinate synthetase A
Pr62.2Low levels in gametes, higher in zygotes0.35d68d
Pr72.2All stages
Pr92.3All stages
BG860484NDVegetative; ND for gametes, zygotesESTs BG860484, BG859735, and BG856733 match the AC29 genomic sequence adjacent to the predicted Ac29 gene
Ac292.5Vegetative; reduced or absent in gametes, zygotes0.3667Acetate-requiring mutant, yellow color. Similar to the Arabidopsis ALBIN03 gene
Genes in both mating types, R domain
Pr1051.5Vegetative; reduced or absent in gametes, zygotesLocated in segment 1
Pdk2.6Vegetative; no data on gametes or zygotes0.3865Similar to pyruvate dehydrogenase kinase (or to branched-chain α-keto acid dehydrogenase kinase). Located in segment 2
Pr431.5All stagesLocated in segment 2
Pr442.0All stagesLocated in segment 2
Pr46a1.1Vegetative; reduced or absent in gametes, zygotes0.3056Similar to a widely conserved protein of unknown function. Located in segment 3
Pr46b1.4ND, presumed vegetative0.3565Located in segment 3
GdcH1.1Vegetative; reduced or absent in gametes, zygotes0.5563Similar to glycine decarboxylase complex subunit H. Located in segment 3
Pr651.1All stagesLocated in segment 4
Genes in both mating types, C domain
Portion of V-Ezy2spacerNANAΨ1.5 kb of the 16-kb repeat containing the Ezy2gene is at the start of the C domain region (see Figure 10)
Pr713.1Gametes only
Pr721.0Zygotes onlyMay be a duplicate copy of the Pr74 gene
Pr741.0Zygotes onlyMay be a duplicate copy of the Pr72 gene
Pr811.1Vegetative; in lesser amounts in gametes, zygotes
Sadl12minus gametes only, some remain in 30-min zygotesminus agglutinin gene
SwampNANASection of multiple tandem repeats of 1.1-kb unit
Pr923.0Gametes only
AV639313NDVegetativeESTs AV639313 and AV634214 match the THI10 genomic sequence adjacent to the predicted Thi10 gene
Thi10NDND, presumed vegetative0.4770Hydroxyethylthiazole kinase gene, predicted from genomic sequence (K. Shimogawara, personal communication)
Mat34.5All stages (J. G. Umen, personal communication)0.3868Retinoblastoma protein homolog
Ezy12.2Zygotes only0.44d71Zygote-specific protein that localizes to chloroplast nucleoids; multigene family
Pr972.2Zygotes only
Pr983.0Gametes and zygotes
Pr991.3Vegetative; in lesser amounts in gametes, zygotes
Pr1006.5Zygotes onlyProbe 100 is derived from an inverted tandem duplication
Fa15.8Vegetative; no data on gametes or zygotes0.3375Flagellar autotomy gene
Genes only in mating type minus
Ψ-Ezy2NANAA single pseudogene copy of Ezy2
Mid1.1minus gametes0.1650minus dominance gene
Mta12.2minus gametes and faintly in 30-min zygotes0.3068
Genes only in mating type plus
Fus13.0plus gametes0.0548plus-specific fusion gene
Mta10.8plus gametes and 30-min zygotes, less in 3-hr zygotes0.4665
Ψ-Mta2NANAPseudogene copy of the HRGP Mta2 gene
Ψ-Mta3NANAPseudogene copy of the Mta3 gene
TOC2NANAA TOC2-like element (shown in Figure 5 only)
GulliverNANAA Gulliver-like element (shown in Figure 5 only)
Ezy23.9Zygotes only0.3568Tandemly repeated gene family
Gulliver ONDNDA copy of the Gulliver transposon, present at this position in strain CC-620 only
  • ND, not determined; NA, not applicable.

  • a B value is a measure of codon usage bias (Long and Gillespie 1991). A B value of 0 indicates that all codons are used equally frequently; a value of 1 indicates that only one codon is used for each amino acid.

  • b Calculated for the coding region only.

  • c Based on partial gene sequence.

  • d Calculated for pr6(−) allele.

  • e Calculated for L20946.