Frequency of heterokaryon formation
| Strain | Viable conidia | Reference strain | Viable conidia | No. of colonies | Frequency |
|---|---|---|---|---|---|
| 9-1-5 | 60 | FGSC 4564 | 1.3 × 105 | 16 ± 1 | 1 |
| 8-88 | 1.2 × 105 | FGSC 4564 | 1.3 × 105 | 2 ± 1 | 6.2 × 10−4 |
| Cr1-10 | 1 × 105 | FGSC 4564 | 1.3 × 105 | 0–1 | 2.5 × 10−4 |
| 8-88 (Xhk5-4) | 1 × 105a | FGSC 4564 | 1.3 × 105 | 41 ± 8 | 1.6 × 10−3 |
↵a Due to the heterokaryotic nature of transformants in N. crassa, the conidial concentration of a 8-88 (Xhk5-4) transformant was determined by counting the number of conidia under the microscope via a hemacytometer rather than by assessing viable conidia by plate counts.