TABLE 2

Frequency of heterokaryon formation

StrainViable conidiaReference strainViable conidiaNo. of coloniesFrequency
9-1-560FGSC 45641.3 × 10516 ± 11
8-881.2 × 105FGSC 45641.3 × 1052 ± 16.2 × 10−4
Cr1-101 × 105FGSC 45641.3 × 1050–12.5 × 10−4
8-88 (Xhk5-4)1 × 105aFGSC 45641.3 × 10541 ± 81.6 × 10−3
  • a Due to the heterokaryotic nature of transformants in N. crassa, the conidial concentration of a 8-88 (Xhk5-4) transformant was determined by counting the number of conidia under the microscope via a hemacytometer rather than by assessing viable conidia by plate counts.