TABLE 8

Examples of border sequence assignments to genome locations

Consensus sequence lengthdGenome sequence identity
Line no.aAccession no.bInsert positioncBest matcheNext matchf
14986AP00041051816631538/54920/20
54505690572/57222/23
55132AL13865015928761645/64519/19
15953773643/64322/22
29862AL16229554121496421/42320/20
54134765683/68720/20
01917AL161565901014791299/134456/61
90111771743/746261/282
16713AB023039460371031894/90386/104
46227673624/62627/28
36172AB01148251071739641/64419/19
51981738645/64826/28
29863AC025417103077660528/55021/22
103096388309/31418/18
00978AC01697258221634589/58918/18
58301425394/39463/68
18048AC00643966380624486/48825/26
66394606416/41918/18
18247AC0072906171666517/51918/18
6194238153/15619/19
21157AC00692960173338231/23221/21
60189601521/52329/32
07724AC00238765413483391/391128/132
65432726640/642110/113
  • a Reference number for mutant in Syngenta collection.

  • b Contains best border sequence match and provides framework for insertion site coordinates.

  • c Coordinate numbers decrease from insertion site for first border and increase from insertion site for second border.

  • d Edited sequence from TAIL-PCR products, plasmid rescue inserts, adaptor or direct PCR products, or some combination thereof. Differs from number of bases in best match, identified from BLAST output, due to presence of vector sequences at product ends.

  • e Number of sequence identities with the assigned locus/number of bases identified from BLAST output.

  • f Number of sequence identities with a separate locus/number of bases identified as next best sequence match.