TABLE 3

Expected response of seedlings on selection medium

Total insertsaMutant taggedUnlinked insertsbRatio R:S seedlingsc% EMB/EMB transplantsd
1Yes020
2Yes11127
3Yes24732
1No1333
2No21533
3No36333
  • Parental (T1) lines are assumed to be heterozygous for an emb mutation that prevents survival of homozygotes.

  • a Number of functional insertion sites in parental line.

  • b Unlinked relative to EMB locus.

  • c Expected ratio of resistant to sensitive seedlings on selection medium.

  • d Percentage of resistant (T2) transplants that should appear wild type.