TABLE 1

Oligonucleotide sequences for TAIL-PCR

NameSequence 5′ → 3′Utility
LB1aGCC TTT TCA GAA ATG GAT AAA TAG CCT TGC TTC C1° TAIL
LB2aGCT TCC TAT TAT ATC TTC CCA AAT TAC CAA TAC A2° TAIL
LB3aTAG CAT CTG AAT TTC ATA ACC AAT CTC GAT ACA C3° TAIL, sequencing
RB1bATT AGG CAC CCC AGG CTT TAC ACT TTA TG1° TAIL
RB2bGTA TGT TGT GTG GAA TTG TGA GCG GAT AAC2° TAIL
RB3bTAA CAA TTT CAC ACA GGA AAC AGC TAT GAC3° TAIL, sequencing
QRB1cCAA ACT AGG ATA AAT TAT CGC GCG CGG TGT CA1° TAIL
QRB2cGGT GTC ATC TAT GTT ACT AGA TCG GGA ATT GA2° TAIL
QRB3cCGC CAT GGC ATA TGC TAG CAT GCA TAA TTC3° TAIL, sequencing
SKI1dAAT TGG TAA TTA CTC TTT CTT TTC CTC CAT ATT GA1° TAIL
SKI2dATA TTG ACC ATC ATA CTC ATT GCT GAT CCA T2° TAIL
SKI3dTGA TCC ATG TAG ATT TCC CGG ACA TGA A3° TAIL, sequencing
AD1aeTGW GNA GSA NCA SAG ATAIL-PCR, TAIL2K
AD1fNTC GAS TWT SGW GTTTAIL-PCR, TAIL2K
AD2fNGT CGA SWG ANA WGA ATAIL-PCR, TAIL2K
AD2gAGW GNA GWA NCA WAG GTAIL-PCR, TAIL2K
AD3fWGT GNA GWA NCA NAG ATAIL-PCR, TAIL2K
AD5hSTT GNT AST NCT NTG CTAIL-PCR, TAIL2K
  • a For left border region of pCSA104, pCSA110, and pDAP101.

  • b For right border region of pCSA104 and pDAP101.

  • c For right border region of pCSA110.

  • d For left border region of pPCVICEn4HPT and pSKI015.

  • e A modified AD1 as compared to Liu and Whittier (1995).

  • f Liu et al. (1995).

  • g Liu and Whittier (1995).

  • h Tsugeki et al. (1996).